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Basic Acquisition Methods
System User Guide
D5033331 A
AB SCIEX TripleTOF
®
5600/5600+ Instruments
43 of 116
Add an Experiment
1. Right-click the period where an experiment needs to be added and then click Add
experiment.
An experiment is added below the last experiment in the period.
2. In the Acquisition Method Editor pane, select the appropriate device or instrument
parameters.
Add a Period
• In the Acquisition method pane, right-click the Mass Spec icon, and then click Add
period.
A period is added below the last period created.
Copy an Experiment into a Period
Prerequisite: Multi-period method
•In the Acquisition method pane, press CTRL, and then drag the experiment to the
period.
The experiment is copied below the last experiment in the period.
Copy an Experiment within a Period
Use this procedure to add the same or similar experiments to a period if most or all of the
parameters are the same.
• Right-click the experiment and then click Copy this experiment.
A copy of the experiment is added below the last experiment created. This is useful
when the same or similar experiments are added to an acquisition method.
Note: An experiment or a period cannot be inserted between experiments
or periods. Users can only add an experiment at the end of the period.
Note: Users cannot use multiple periods in an IDA experiment.
- AB SCIEX TripleTOF 1
- 5600/5600+ 1
- Instruments 1
- Contents 3
- General Safety Information 7
- Symbols and Conventions 7
- Qualified Personnel 8
- Regulatory Compliance 10
- International 11
- IN DIAGNOSTIC PROCEDURES 12
- Mains Supply 14
- Laboratory Ventilation 15
- Environmental Conditions 15
- Electronic Equipment) 16
- System Information 17
- Theory of Operation 17
- Data Handling 17
- System Overview 18
- Start Up the System 20
- Shut Down the System 21
- Instrument Safe Fluids 26
- Sample Workflows 27
- Hardware Profiles 29
- Create a New Subproject 36
- Copy a Subproject 36
- Installed Project Folders 37
- Optimize the Instrument 39
- Basic Acquisition Methods 41
- Add an Experiment 43
- Add a Period 43
- Scan Techniques 44
- Single Mass Spectrometry 44
- Tandem Mass Spectrometry 44
- About Instrument Parameters 45
- Create and Submit a Batch 51
- Set up Sample Calibration 54
- Acquire Data 55
- (Optional) 57
- Stop Sample Acquisition 57
- Batch Editor Right-Click Menu 58
- Queue States 59
- Queue Right-Click Menu 62
- Analyzing and Processing Data 65
- Show Experimental Conditions 66
- Show ADC Data 67
- Chromatograms 68
- Show TICs from a Spectrum 69
- Show a Spectrum from a TIC 69
- Generate XICs 69
- Generate BPCs 72
- Generate XWCs 74
- View DAD Data 74
- Generate TWCs 74
- Adjust the Threshold 75
- Data Processing 77
- Zoom in on the y-axis 79
- Zoom in on the x-axis 79
- TurboIonSpray Probe 84
- APCI Probe 85
- Ion Source Latch 86
- Source Exhaust System 86
- Installation 87
- Install the Ion Source 88
- Optimization 89
- Run the Method 90
- Set the Starting Conditions 90
- Optimize the APCI Probe 92
- Maintenance 95
- Remove the Ion Source 96
- Remove the Probe 96
- Clean the Electrode Tube 97
- Replace the Electrode 98
- 0.5 - 1 mm 99
- Replace the Sample Tubing 100
- Troubleshooting 101
- Consumables 102
- Cleaning and Maintenance 103
- Health and Safety Precautions 103
- Clean the Surfaces 104
- Empty the Drain Bottle 104
- Front-End Cleaning 105
- Best Practices 106
- Prepare for Routine Cleaning 107
- Clean the Curtain Plate 108
- Basic System Troubleshooting 111
- Recommended Calibration Ions 113
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