Axis BTA 2100D Manual do Operador descarregar pdf (Página 100)

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temperatures and this is okay. In general use the lowest temperature which

provides acceptable chromatography. The manifold bakeout functions can be

used to do this overnight and reset the temperatures to operating conditions

automatically.

The trap temperature value selected should be high enough so that

chromatographic performance is not affected. We have found trap temperatures

can be 50-75°C below the ending temperature for the GC column temperature

ramp, without compromising chromatographic performance.

Certain compounds (e.g., straight chain hydrocarbons) will show more

fragmentation at hotter ion trap temperatures.

AGC Target TIC Value

In EI mode with AGC on, the target TIC value determines how many ions AGC

allows into the ion trap during the calculated ionization time. While increasing the

target TIC value will increase peak heights, increasing it too far will result in a

loss of mass resolution. This would be observed as mass misassignments and/or

incorrect isotope abundance ratios. For full-scan EI operation, a target TIC of

20,000 is a good starting point. Under special circumstances, e.g., MS/MS, a

much lower target TIC is required. As the m/z distribution of the stored ions

becomes smaller, fewer ions can be trapped without a loss of resolution.

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