Axis BTA 2100D Manual do Operador descarregar pdf (Página 212)

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multiple chemical bonds or that undergo significant rearrangements. Periodic

modulation through ion resonance results in an averaging of the energy coupled

into the ion motion and provides a reproducible product ion spectrum, even with

changes in concentration. A disadvantage of this method is that two parameters

in addition to the excitation time and CID excitation amplitude need to be

optimized; these are the modulation range and CID bandwidth.

Changing the CID bandwidth permits simultaneous excitation of ions within a

range of masses. As CID bandwidth increases, the corresponding mass range

also increases. At a given CID bandwidth, mass range increases with increasing

ion mass, and decreases with increasing excitation rf storage level.

Excitation rf Storage Level (m/z): 48

CID Bandwidth

()

Mass

Range

0 2 4 6 8 101214161820

m/z=500 m/z=400 m/z=300 m/z=200 m/z=100

Mass range vs. CID bandwidth for an excitation rf storage level of 48 m/z

Excitation rf Storage Level (m/z): 143

CID Bandwidth

Mass

Range

0 2 4 6 8 101214161820

m/z=500 m/z=400 m/z=300 m/z=200

Mass range vs. CID bandwidth for an excitation rf storage level of 143 m/z

1 2 ... 207 208 209 210 211 212 213 214 215 216 217 ... 596 597

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