Axis BTA 2100D Manual do Operador descarregar pdf (Página 214)

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level of parent or product ions in the analytical scan results in consistent,

reproducible product ion spectra.

Lower target values may be required with the MS/MS option to prevent space

charging effects. Based on the isolated parent ion, the following target values are

recommended.

Parent Ion Target

60  m/z  150

10000

150  m/z  500

5000

500  m/z  650

2000

Additionally, the EI-Auto PreScan ionization time must be changed from the

default value. This is done automatically when the user selects MS/MS ion prep.

The PreScan ionization time is changed to 1500 µsec.

The MS/MS Ion Prep Method

MS/MS Software Parameters

Under the Isolation header, enter the Parent Ion Mass (m/z). The parent ion

mass range is 50 to 650 m/z.

NOTE: Integer mass values may be used if the mass isolation window (next

step) is greater than 1.5 m/z. If the window size is less than 1.5 m/z, or if the

mass defect of the parent ion is greater than approximately 0.3 m/z, use the

exact fractional mass.

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