Axis BTA 2100D Manual do Operador descarregar pdf (Página 224)

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It is a good idea to verify that the parent ion is isolated without a large loss in

intensity. This can be done by setting the excitation amplitude for scan segment

1=0 volts. Inject the sample and examine the spectra across the peak

Set the excitation rf equal to the lowest mass product ion expected divided by

1.4. If the product ions are not yet known, start with the excitation rf=48 m/z and

an excitation time of 20 ms. Set up the AMD method to cycle through excitation

voltages using increments of 5 or 10 volts.

NOTE : If you require a more accurate value for this voltage, you may inject the

sample and increase the CID (excitation) amplitude by 2 or 3 volts for each group

in the vicinity of the best values found with the 10-volt increments.

NOTE: If the rf level has been raised to trap the lowest product ion and a

satisfactory number of product ions still has not been obtained, either try another

parent ion or switch to resonant excitation. Some ions are sufficiently stable that

they are ejected from the trap before they can acquire enough energy to

dissociate. You may also try increasing the excitation time.

Determine the optimum voltage by plotting the product ion intensity data as a

function of CID (excitation) amplitude as shown below.

Nonresonant Excitation of a Typical Ion

Excitation amplitude (volts)

200

400

600

800

1000

1200

0 102030405060708090100

Parent ion

Product ion 1

Product ion 2

1 2 ... 219 220 221 222 223 224 225 226 227 228 229 ... 596 597

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