Axis BTA 2100D Manual do Operador descarregar pdf (Página 225)

100

101

223

Determine the optimum rf level by plotting the product ion intensity data as a

function of CID (excitation) amplitude for different rf levels as shown below.

Product Ion Formation vs. Excitation RF for a Typical Ion

Using Nonresonant Excitation

Excitation amplitude (volts)

100

200

300

400

500

600

700

800

900

1000

0 102030405060708090100

RF=48 m/z RF=63 m/z RF=95 m/z

NOTE: The maximum yield of product ions shifts to higher CID (excitation)

amplitudes as the rf level is increased.

Determining the Optimum Voltage for Resonant

Excitation Using Automated Methods Development

(AMD)

Follow these steps to determine the optimum voltage for resonant excitation

using AMD while injecting the parent compound.

Set the excitation rf equal to the lowest mass product ion expected divided by

1.4. If the product ions are not yet known, start with the excitation rf=48 m/z and

an excitation time of 20 ms. Set up the AMD method to cycle through excitation

voltages using increments of .2 or .5 volts.

Determine the optimum voltage by plotting the product ion intensity data as a

function of CID (excitation) amplitude as shown below.

Resonant Excitation of a Typical Ion

Excitation amplitude (volts)

200

400

600

800

1000

1200

01234567

Parent ion

Product ion 1

Product ion 2

This figure shows a typical breakdown curve for resonant excitation using default

modulation parameters.

1 2 ... 220 221 222 223 224 225 226 227 228 229 230 ... 596 597

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