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MS
n
MS
n
uses two parent ions for sequential dissociation. The additional dissociation
step can be used to increase selectivity of analyte versus background or for
additional structure information.
First, work out optimized conditions for MS/MS of the first parent ion. AMD will
make this process go faster.
Then optimize the conditions for dissociation of that product ion (entered under
parent ion 2). You may need to make sequential injections using the MS
n
method. If the second generation product ion is in the original EI or CI spectrum,
then you can use AMD to find the optimum conditions.
Item Description
Parent Ion Mass (m/z) Integer or exact mass. Parent ion 1 mass range
is 50 to 650 m/z. Parent ion 2 mass range is
from 50 to (parent ion-1)m/z, and so on.
Isolation Window (m/z) Integral and fractional mass isolation windows
are both accepted. Range is 1.0 to 14.0 m/z
and is mass dependent. The default value is
3.0 m/z.
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