Axis BTA 2100D Manual do Operador descarregar pdf (Página 207)

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Tips for Using SIS

Observe these recommendations for best results when using the SIS application.

 When selectively storing ions that are easily fragmented by collision-induced

dissociation (CID), use wide mass isolation ranges and low amplitude

waveforms.

 As the frequency notches become increasingly narrow and the voltage of the

frequency components increases, the ions that you wish to store acquire

sufficient energy to dissociate or be ejected. As a result, a small fraction of

these ions may be lost due to dissociation or ejection.

 Target TIC values less than 10,000 are recommended when using SIS

applications, especially with a narrow mass window. Values in excess of

10,000 may cause space charge build-up, which can decrease the linear

range for quantitation and result in loss of resolution.

The MS “q” Calculator

For convenience, The MS q Calculator is available in all Ion Preparation Options.

Storage rf values are often reported in terms of the Mathieu “q” parameter in the

scientific literature. The value of the “q” parameter determines the stability of the

parent ion trajectory. More stable trajectories allow higher excitation voltages to

be applied before ions are ejected from the trap. It has been empirically observed

that a “q” value of 0.4 provides an optimum yield of product ions. This calculator

utility determines the corresponding CID storage rf value (m/z) following user

entry of an ion mass (m/z) and desired “q”.

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